poson insertions were distributed at 43 sites in the gene ftsK.

umbers of insertions in two genes ended up with very different

of insertion sites. This thus shows that using the site statistic,

A1 is very likely identified as an essential gene. However, when

insertion statistic, it is very likely both genes will be classified

ame category, being either essential or non-essential because they

similar transposon insertions.

he insertion spectrum for the gene rpoA1 of the first replicate of the Francisella

species.

The insertion spectrum for the gene ftsK of the first replicate of the Francisella

species.

has led to new and more robust transposon statistic which is the

on between the site statistic with the insertion statistic. It is called

ation feature statistic (MF) [Yang, et al., 2017]. During a

on process, the essentiality function of the transposon insertion

both distal ends of a gene is penalised. An insertion site near the

sition of a gene is assigned a greater weight while an insertion

either distal end of a gene is assigned a smaller weight. Therefore,

an summing up insertions across a gene as in ESSENTIALS

et al., 2012] or ignoring the site impact on gene essentiality as in

Langridge, et al., 2009], MF sums up the insertions within a gene